PUROMYCIN DIHYDROCHLORIDE CAS 58-58-2

Table of Contents

• Product Overview & Specifications
• Detailed Applications in Research
• Step-by-Step Usage Protocol
• Industry Application Case Studies
• Verified Client Success Stories
• Contact for Technical Consultation

Puromycin Dihydrochloride CAS 58-58-2: Technical Specifications

Key Parameters

Comparative Analysis

Research Applications

Core Functions

• Stable cell line selection (eukaryotic systems)
• Translation termination in pulse-chase experiments
• Antibiotic selection in CRISPR-edited cells

Experimental Scenarios

• Mammalian cell transfection validation
• Ribosome profiling studies
• Inducible expression system optimization

Experimental Protocol

Standard Workflow

1. Prepare 10 mg/mL stock solution in sterile PBS
2. Filter-sterilize using 0.22 µm membrane
3. Apply 2-5 µg/mL final concentration to culture medium
4. Monitor cell viability for 72 hours

Critical Notes

• Light-sensitive: protect from prolonged exposure
• Effective pH range: 6.0-7.5
• Not suitable for plant cell systems

Documented Applications

Case 1: Stable CHO Cell Line Development

Biopharma Inc. achieved 95% transfection efficiency using 3 µg/mL puromycin selection over 10-day period for monoclonal antibody production.

Case 2: Viral Vector Packaging Optimization

Gene Therapy Labs reduced selection timeline from 14 to 7 days through pulsed puromycin treatment (5 µg/mL, 48h intervals).

Client Implementation Records

University Research Team

• Institution: Stanford Cell Biology Department
• Application: CRISPR-Cas9 edited HEK293T cell selection
• Result: 98% pure population in 96 hours

Biotech Startup

• Company: NeoBiotechnologies
• Application: High-throughput drug screening models
• Result: Reduced false positives by 40% vs. alternative antibiotics

Technical Support & Ordering

For protocol optimization or bulk purchasing:

• Email: info@vivalr.com
• Phone: (86) 15866781826

Why Choose Our Product:

• Batch-to-batch consistency (HPLC certified)
• Custom concentration formulations available
• GMP-grade production options