Contact Us Today to Schedule a Free Sample !

Specialized inDisodium 4-Nitrophenylphosphate CAS 4264-83-9and related product development and production

Chemical Name: Disodium 4-Nitrophenylphosphate
Synonyms: 4-Nitrophenyl phosphate disodium salt; 4-Nitrophenyl disodium orthophosphate; PNPP
CAS No.: 4264-83-9
Molecular Formula: C6H7NNaO6P
Molecular Weight: 243.09
Appearance: Flaky

Samples: Available / Stock: Sufficient
Estimated delivery time: 3-5 days

Disodium 4-Nitrophenylphosphate (CAS 4264-83-9) User Guide

1. Product Specifications & Comparative Analysis

Parameter Specification
CAS No. 4264-83-9
Molecular Formula C6H4NNa2O6P
Molecular Weight 263.05 (Anhydrous)
371.14 (Hexahydrate)
Appearance White to pale yellow crystalline powder
Melting Point 300°C
Solubility Water-miscible (1 tablet dissolves in H2O)
Stability Light/moisture-sensitive; decomposes above 25°C
Absorption Peak 310 nm (native), 405 nm (reaction product)

Comparative Advantages vs. Similar Substrates

Feature PNPP OPD TMB
Sensitivity High (405 nm detection) Moderate High
Reaction Termination 3N NaOH H2SO4 Acid
Storage -20°C, desiccated 4°C RT

2. Applications & Functional Mechanisms

Primary Use: Chromogenic substrate for alkaline/acid phosphatase assays in:

  • ELISA-based diagnostic kits
  • Pharmaceutical QC for enzyme activity validation
  • Biochemical research (enzyme kinetics, inhibitor screening)

Reaction Workflow

  1. Substrate hydrolysis by phosphatases releases p-nitrophenol
  2. Yellow coloration intensity correlates with enzyme activity
  3. Quantification at 405 nm with linear range 0.1-10 mM

3. Operational Guidelines & Best Practices

Protocol Summary

Step Condition Note
Reconstitution 1 mg/mL in assay buffer Prepare fresh
Incubation 37°C, 5-30 min Monitor color development
Termination 3N NaOH (1:1 v/v) Stabilizes signal for 2h

Critical Handling Notes

  • Avoid repeated freeze-thaw cycles
  • Use amber vials for light-sensitive storage
  • Discard precipitated solutions

4. Industry Use Cases

Diagnostic Manufacturing

Integrated into automated immunoassay platforms for:

  • Hepatobiliary disease panels
  • Bone metabolism marker tests

Biopharma R&D

High-throughput screening of phosphatase-targeted drug candidates with:

  • Z´ factor >0.7 in 384-well formats
  • CV <8% across replicates

5. Client Success Stories

Case 1: European Pharma Leader

  • Challenge: Unstable phosphatase signals in lead optimization
  • Solution: PNPP-based kinetic assays with real-time monitoring
  • Outcome: 40% reduction in false positives

Case 2: Asian CRO

  • Challenge: Batch variability in clinical trial kits
  • Solution: Standardized PNPP lots with COA tracking
  • Outcome: 99.2% inter-assay consistency achieved

6. Technical Support & Inquiry

Request bulk pricing, COA, or formulation guidance:

Email: info@vivalr.com
Tel: (86) 15866781826

弹出框

Request a Free Quote

The Manager has Something to Say

This is William, CEO of fousi chemical Chemical Co., Ltd.

Welcome to visit our factory . As the top chemical manufacturer, we have been striving for product quality, innovation , R&D, and customer service for the past 7 years. In the next 10 years or even longer, we are committed to becoming the most reliable chemical supplier in the world , creating a well-known international brand trusted by customers, with the spirit of “one meter wide, ten thousand meters deep”, we continue to focus on product research and development, continue to focus on customer service, continue to improve the supply chain service system, to create a professional chemical supply service team, to achieve win-win long-term cooperation. Please feel free to contact us if you have any questions.

Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
National high-tech enterprise
5000+ square meters R&D center
A 70+ R&D team led by Chinese and foreign doctors
150 pieces (sets) of R&D equipment, 120+ fume hoods
18 patents

Solid QMS. Offer top-quality products and services.

Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
It has successfully passed on-site inspections from the United States, Australia, Poland, Japan and domestic pharmaceutical company customers and institutions many times, providing high-quality one-stop services for R&D and production of new drugs around the world.

More than 150 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

About the company

International Standard Chemicals Sell To All Over The World

FAQ

Does it support auditing?

Support, please make an appointment one day in advance. Company address: Room 716, Building 3, Ligao International Garden, No.1222 West Aoti Road, Lixia District, Jinan City, Shandong Province, P.R. China

Whether to provide samples?

Free samples can be provided for low value products, and small packaging specifications can be purchased for high value products.

Delivery time

Under normal circumstances, all items are in stock and will be shipped before 4 o'clock on the same day. Some products are produced to order, depending on the workshop situation and production schedule.

What is the production capacity?

At present, the company's workshop production equipment is mainly based on laboratories and pilot lines, with a maximum daily production capacity of 1.5 tons.

Can additional test indicators be added?

We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Chem-iso’s delivery rate is guaranteed to be 99.99%

5000+

square meter R&D center

150+

China(set) of R&D equipment

150+

Fume hood

180+

R&D team

User’evaluation of us

"Chem-ios is my partner for years. Met at 2014 Shanghai CPHI Expo. Not interested then as had stable supplier. In Sep 2016, original supplier couldn't guarantee quality and delivery. Thought of Zhai and his company first. So far, have stable business relationship and good personal friendship."

Jasmine Hook

" In years of sourcing from China, first found working with Sincere Chemical great. They help in production, delivery and tech, saving time. When first asking Zhai for optical brightener quote, got detailed one with prices, trade details, company info, R&D and production capacity, packaging and raw material control."

Michael Cooper

" Hi, I'm Kim, Zhai's partner. Don't like him much (as he never gives more for my prices, haha), but still write customer feedback. Zhai's emails help me understand product and industry. Satisfied with his work ethic. He provides quotes and helps with work details, so I can focus on other things. He's the best supplier for me."

James Black

" Sincere Chemical team visited our USA factory in the year of 2017. We think highly of cooperation with them. We thanked you for the past excellent orders and best product quality. We also talked about some new products for future orders. We will be still in strategic cooperation in the future and help each other."

Lisa Goodwill

" I just got the product. The quality is awesome. I’m very happy. Hope to place soon a new order in your company. Also wish for our future business."

Ashton Spagg

"Sincere Chemical is reliable and professional. Its team is excellent in exporting and service, giving suggestions for our new project. Satisfied with service and product quality. Vendor code: 51465768."

Margo Brown

" I have visited Sincere Chemical company in the year 2018. Their company can be considered as one of the most professional, customer-focused, and product quality-driven companies that I have worked with in recent years."

Leo Rodgers

" We are a pharmaceutical manufacturer in Brazil. Happy to cooperate with Sincere Chemical, especially with Zhai. He is patient and friendly. Satisfied with his 24/7 service. We are not only cooperators but also best friends."

Milla Yoshi

Request A Free Quote

弹出框

Request a Free Quote

databaseflagenvelopegraduation-hatcartheart-pulserocketalarmclockthumbs-up