Chemical Name: Coumarin 6
CAS No.: 38215-36-0
Molecular Formula: C20H18N2O2S
Molecular Weight: 350.43
Parameter | Coumarin 6 (CAS 38215-36-0) |
---|---|
Chemical Name | 3-(2-Benzothiazolyl)-7-(diethylamino)coumarin |
CAS Number | 38215-36-0 |
Molecular Formula | C20H19N2O2S |
Molecular Weight | 350.44 g/mol |
Appearance | Yellow-green crystalline powder |
Purity | ≥98% (HPLC) |
Solubility | DMSO, DMF, Chloroform |
Storage | 2-8°C in dark conditions |
Preferred for cellular membrane studies due to exceptional quantum yield (0.78-0.85) and Stokes shift (~90 nm).
Superior photostability enables long-term tracking in:
- Live cell imaging
- Subcellular structure analysis
- Drug delivery visualization
Feature | Coumarin 6 | Rhodamine B | Fluorescein |
---|---|---|---|
Excitation (nm) | 460 | 540 | 494 |
Emission (nm) | 505 | 625 | 521 |
Quantum Yield | 0.82 | 0.31 | 0.95 |
Beijing Institute of Nanotechnology achieved 93% tracking accuracy in polymeric nanoparticles using 0.01% w/w Coumarin 6 loading.
Challenge: Required low-toxicity probe for 72-hour continuous neuron imaging
Solution: 50μM Coumarin 6 in PBS buffer
Outcome: Achieved 89% cell viability after 72h exposure
Requirement: Real-time monitoring of micelle drug release
Implementation: 0.05% Coumarin 6 encapsulation
Result: Quantified 82% payload release within 48h
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