Thiazolyl Blue CAS 298-93-1
Chemical Name: Thiazolyl Blue
Synonyms: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide; Thiazolyl Blue Tetrazolium Bromide; MTT
CAS No.: 298-93-1
Molecular Formula: C18H16BrN5S
Molecular Weight: 414.32
Appearance: Powder
Thiazolyl Blue (CAS 298-93-1) Comprehensive Product Guide 2025
Table of Contents
- 1. Product Specifications & Comparative Analysis
- 2. Detailed Applications in Biomedical Research
- 3. Operational Protocols & Best Practices
- 4. Industry Application Case Studies
- 5. Client Success Stories
- 6. Professional Consultation & Quotation
1. Product Specifications & Comparative Analysis
| Parameter | Thiazolyl Blue (MTT) | XTT Assay | WST-1 |
|---|---|---|---|
| CAS Number | 298-93-1 | 111072-31-2 | 129830-38-2 |
| Molecular Formula | C18H16BrN5S | C18H13N4NaO13S3 | C22H17N4NaO14S3 |
| Detection Sensitivity | 5×103 cells/well | 1×104 cells/well | 2×103 cells/well |
| Solubility Requirement | DMSO required | Aqueous solution | Direct measurement |
2. Detailed Applications in Biomedical Research
Core Applications:
- Cell proliferation quantification in cancer research
- Cytotoxicity assessment for drug development
- Bioactivity screening of natural compounds
- Nanomaterial safety evaluation
Technical Advantages:
- 94.7% correlation with ATP-based assays
- Stable formazan crystals for reliable spectrophotometric measurement
- Compatibility with 96-well/384-well microplates
3. Operational Protocols & Best Practices
Standard Protocol:
- Prepare 5 mg/mL stock solution in PBS
- Add 20μL reagent per 100μL culture medium
- Incubate 3-4 hours at 37°C with 5% CO2
- Add solubilization solution (10% SDS in 0.01M HCl)
- Measure absorbance at 570nm with 630nm reference
4. Industry Application Case Studies
Pharmaceutical Development:
Used in Phase II trials of novel kinase inhibitors (2023 data):
- IC50 determination accuracy: ±5% vs flow cytometry
- Throughput: 200 compounds/day screening capacity
5. Client Success Stories
Case 1: Shanghai BioPharm Co., Ltd.
- Application: High-throughput screening of TCM extracts
- Outcome: Identified 3 lead compounds with 80% cytotoxicity selectivity
- Throughput improvement: 300% vs previous Alamar Blue method
Case 2: National Institute of Nanotechnology
- Application: Graphene oxide safety profiling
- Results: Established 50μg/mL as safe exposure threshold
- Validation: 98% correlation with LDH release assay
6. Professional Consultation & Quotation
Contact our technical experts for:
- Bulk pricing (GMP-grade available)
- Custom formulation services
- Assay development support
Email: info@vivalr.com
Tel: (86) 15866781826
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