Fuchsine Basic (CAS 632-99-5): Comprehensive User Guide
Table of Contents
1. Product Overview & Specifications
Fuchsine Basic (CAS 632-99-5), also known as Basic Violet 14, is a triarylmethane dye widely used in biological staining and industrial applications.
| Parameter | Fuchsine Basic | Similar Dyes (e.g., Crystal Violet) |
|---|---|---|
| Chemical Formula | C20H20ClN3 | C25H30ClN3 |
| Molecular Weight | 337.85 g/mol | 408.00 g/mol |
| Solubility | Water: 50 g/L (20°C) | Water: 10 g/L (20°C) |
| Purity Grade | ≥99% (HPLC) | ≥95% |
2. Key Applications
2.1 Microbiology Staining
Used in Gram staining to differentiate bacterial species (e.g., Streptococcus identification).
2.2 Textile Industry
Dyeing silk and wool fabrics with superior color fastness (up to 5,000 industrial wash cycles).
3. Usage Protocol
- Prepare 0.1% w/v solution in distilled water
- Fix samples with heat or methanol
- Stain for 60-120 seconds
- Rinse with acidic alcohol (1% HCl in 70% ethanol)
4. Implementation Examples
4.1 Clinical Lab Workflow
City Hospital (Berlin) reduced false-positive Gram stains by 22% using our standardized Fuchsine solution.
5. Verified Customer Results
Case 1: BioTech Labs GmbH
Challenge: Inconsistent fungal hyphae visualization
Solution: Implemented Fuchsine Basic (0.2% in PBS)
Outcome: 40% improvement in microscopy resolution
6. Get Expert Support
Contact our dye specialists for technical queries or bulk orders:
Email: info@vivalr.com
Tel: (86) 15866781826
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