Sulforhodamine B (CAS 3520-42-1): Comprehensive User Guide
Table of Contents
1. Product Overview & Specifications
Sulforhodamine B (CAS 3520-42-1) is a red-fluorescent xanthine dye widely used in biochemical and industrial applications. Key parameters:
| Parameter | Sulforhodamine B | Rhodamine B | FITC |
|---|---|---|---|
| Molecular Weight | 580.67 g/mol | 479.02 g/mol | 389.38 g/mol |
| Excitation/Emission | 565 nm/586 nm | 540 nm/625 nm | 494 nm/518 nm |
| Quantum Yield | 0.85 | 0.65 | 0.93 |
2. Key Applications
- Biomedical Research: Cell viability assays, protein labeling
- Industrial Testing: Dye tracing in hydraulic systems
- Environmental Monitoring: Water flow analysis
3. Usage Guidelines
Protocol for Cell Viability Assay:
- Prepare 1 mg/mL stock solution in PBS
- Incubate cells with 50 μg/mL dye for 4 hrs
- Measure fluorescence at 586 nm
4. Case Studies
Case 1: Drug Discovery Screening
A pharmaceutical company achieved 98% accuracy in high-throughput screening using Sulforhodamine B-based cytotoxicity assays.
5. Client Success Stories
Client A: BioTech Solutions Co.
- Challenge: Needed stable fluorescent tracer for wastewater analysis
- Solution: Implemented Sulforhodamine B at 10 ppm concentration
- Result: 40% improvement in detection sensitivity
6. Request a Quote
Contact our technical team for bulk pricing or custom formulations:
Email: info@vivalr.com
Phone: (86) 15866781826
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