3-(N-Morpholino)propanesulfonic Acid Hemisodium Salt (CAS 117961-20-3) Comprehensive Guide
Table of Contents
1. Product Overview & Specifications
CAS No.: 117961-20-3
Molecular Formula: C14H29N2NaO8S2
Molecular Weight: 440.51 g/mol
Appearance: White to off-white crystalline powder
Solubility: Freely soluble in water (0.5 g/mL)
pH Range: 6.5–7.9 (1% aqueous solution)
Comparative Analysis with Related Buffers
| Parameter | MOPS Hemisodium Salt | MOPS Free Acid | HEPES Sodium Salt |
|---|---|---|---|
| Buffering Range | 6.5–7.9 | 6.5–7.9 | 7.2–8.2 |
| Solubility in Water | High | Moderate | High |
| Ionic Strength | Low | None | Moderate |
2. Key Applications
This compound is widely used as a zwitterionic biological buffer in:
- Cell Culture Systems: Maintains pH stability in mammalian cell cultures.
- Protein Electrophoresis: Optimizes separation conditions in SDS-PAGE.
- Molecular Biology: Stabilizes enzymatic reactions (PCR, DNA sequencing).
- Diagnostic Reagents: pH control in clinical assay formulations.
3. Usage Scenarios
Scenario 1: Mammalian Cell Culture Medium Preparation
Dissolve 10 mM MOPS hemisodium salt in DMEM to stabilize pH during CO2-independent incubation.
Scenario 2: Agarose Gel Electrophoresis
Prepare 1× MOPS buffer (40 mM) with 0.1% SDS for RNA separation in denaturing gels.
4. Customer Case Studies
Case Study 1: MedChemExpress (MCE)
Application: Buffer component in kinase assay kits
Volume: 500 g/month
Outcome: Improved assay reproducibility across pH-sensitive reactions.
Case Study 2: Shanghai Yuanye Bio-Technology
Application: Diagnostic reagent formulation
Specification: 99% purity, 500 g batches
Benefit: Enhanced shelf-life stability of pH-dependent reagents.
5. Contact for Inquiries
Request custom specifications or bulk pricing:
| info@vivalr.com | |
| Phone | (86) 15866781826 |
| Lead Time | 2–3 weeks for bulk orders (>10 kg) |
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