Basic Red 2 CAS 477-73-6

Chemical Name: Basic Red 2
CAS No.:477-73-6
Molecular Fomula: C20H19ClN4
Molecular weight: 350.85
Appearance: solid
Assay: ≥99.0%

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Basic Red 2 (CAS 477-73-6): Comprehensive User Guide

1. Product Overview & Specifications

Parameter Basic Red 2 (CAS 477-73-6)
Chemical Name 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride
Molecular Formula C20H19ClN4
Molecular Weight 350.84 g/mol
Appearance Red-brown crystalline powder
Solubility Water: Soluble
Ethanol: Soluble (red solution with yellow fluorescence)
Melting Point >240°C (decomposition)
pH Sensitivity Blue-red in HCl → purple (excess) → blue (large excess)
Green in H2SO4 → blue → purple → red (dilution)
Safety Rating Xi (Irritant) – Handle with PPE

2. Key Applications & Use Cases

Core Functionalities:

  • Biological Staining: Standard dye in histology for nuclei/cartilage visualization
  • Redox Indicator: Visual detection of electrochemical reactions (E0 = +0.24V)
  • Textile Processing: High-affinity cotton/polyester dyeing with ISO 105-C06 compliance
  • Analytical Chemistry: Nitrite detection via diazotization reactions (LOD: 0.1 ppm)

3. Industry-Specific Usage Scenarios

Laboratory Protocols:

Gram Staining (Modified):
1. Fix bacterial smear with heat
2. Apply 0.5% Basic Red 2 solution (60s)
3. Rinse with deionized H2O
4. Counterstain with crystal violet

Industrial Formulation Example:

Textile Dye Bath (1,000L):
– Basic Red 2: 2.5kg
– NaCl: 50kg
– pH Buffer (acetate): 4.5-5.0
– Temperature: 80°C ±2°C
– Process Time: 45min

4. Client Success Stories

Case Study 1: Shanghai JZ Biochemical Co.

Challenge: Required GMP-grade staining agent for vaccine quality control
Solution: Custom 99.9% purity Basic Red 2 in sterile vials
Outcome: 30% reduction in false-positive results during antigen screening

Case Study 2: Guangdong DX Chemical Ltd.

Challenge: Fading issues in automotive upholstery dyes
Solution: UV-stabilized Basic Red 2 formulation
Result: Achieved ISO 105-B02 Grade 5 lightfastness

5. Contact for Custom Solutions

Request technical specifications or bulk quotes:
Email: info@vivalr.com
Tel: (86) 15866781826

Available formats:
– Research Grade (≥98%)
– Industrial Grade (95%)
– Custom Concentrates (5-25% aqueous solutions)

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