Table of Contents
- Product Overview & Specifications
- Key Applications of 3-Morpholinopropanesulfonic Acid (MOPS)
- Industry Use Cases
- Real Client Success Stories
- Request a Quote or Sample
3-Morpholinopropanesulfonic Acid (MOPS) CAS 1132-61-2: Product Overview & Specifications
Basic Parameters
| Parameter | Value |
|---|---|
| Chemical Name | 3-Morpholinopropanesulfonic Acid |
| CAS Number | 1132-61-2 |
| Molecular Formula | C7H15NO4S |
| Molecular Weight | 217.26 g/mol |
| Appearance | White crystalline powder |
| Solubility | Highly soluble in water (>100 mg/mL) |
| pH Range | 6.5-7.9 (pKa = 7.2 at 25°C) |
| Storage Conditions | 2-8°C in dry environment |
| Applications | Biological buffers, molecular biology, cell culture media |
| Grade | Biotechnology Grade (≥99% purity) |
Comparative Analysis with Common Buffers
| Buffer | pH Range | Temp Sensitivity | Metal Chelation | Biocompatibility | Cost Efficiency |
|---|---|---|---|---|---|
| MOPS | 6.5-7.9 | Low | None | High | $$ |
| HEPES | 6.8-8.2 | Moderate | Moderate | High | $$$ |
| Tris | 7.0-9.0 | High | High | Medium | $ |
| PBS | 7.0-7.4 | Low | None | High | $ |
Key Applications of MOPS (CAS 1132-61-2)
1. Biochemical Research
Preferred buffer for electrophoresis due to stable pH under electric fields. Maintains enzyme activity in protein purification.
2. Molecular Biology
Used in RNA isolation (prevents degradation) and cDNA synthesis. Compatible with PCR when magnesium concentration is controlled.
3. Cell Culture Systems
Low toxicity makes it ideal for mammalian cell cultures. Provides consistent osmolarity in media formulations.
4. Diagnostic Reagents
Critical component in IVD kits requiring pH stability at 37°C. Used in blood gas analyzers and immunoassay buffers.
5. Pharmaceutical Formulations
Excipient in lyophilized products. Maintains stability of temperature-sensitive APIs during processing.
Industry Use Cases
Case 1: Protein Purification Optimization
Challenge: Enzyme inactivation during chromatography.
Solution: 20 mM MOPS buffer (pH 7.0) increased yield by 38%.
Result: Reduced aggregate formation in monoclonal antibody production.
Case 2: High-Throughput Screening
Challenge: pH drift in 96-well plates.
Solution: MOPS-based assay buffer maintained ±0.1 pH variance over 24h.
Result: Improved Z’ factor from 0.45 to 0.68 in kinase assays.
Real Client Success Stories
Client A: European Pharma Leader
Project: Vaccine adjuvant development
MOPS Role: Stabilized TLR4 agonist compounds during freeze-thaw cycles
Metrics: 62% reduction in particulate formation
Client B: US Biotech Startup
Project: CAR-T cell expansion media
MOPS Implementation: 50 mM concentration enabled serum-free culture
Outcome: Achieved 95% viability at Day 7 (vs 78% with HEPES)
Client C: Asian Diagnostic Manufacturer
Issue: False positives in lateral flow assays
Fix: MOPS-based conjugate buffer reduced non-specific binding
Impact: Increased test specificity from 88% to 99.2%
Request a Quote or Sample
Contact our technical team for:
- Custom buffer formulation services
- Bulk pricing (25kg+ orders)
- GMP-grade MOPS documentation
Email: info@vivalr.com
Phone: (86) 15866781826
Available: 25g, 100g, 1kg, 10kg packaging



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