UltraPure Agarose (CAS 9012-36-6) – Complete User Guide for Molecular Biology Applications

1. Product Specifications & Comparative Analysis

2. Key Applications in Molecular Biology

2.1 Nucleic Acid Electrophoresis

Optimal for separating DNA fragments from 50 bp to 50 kb with superior band resolution. Compatible with SYBR® Safe and ethidium bromide staining.

2.2 Protein Immunodiffusion

Creates stable matrices for radial immunodiffusion (RID) assays with low background interference.

2.3 Microorganism Cultivation

Ideal for preparing solid media cultures requiring precise nutrient diffusion rates (0.8-1.2% concentration).

3. Operational Protocols & Best Practices

3.1 Gel Preparation Formula

1. Dissolve 1g agarose in 100ml 1X TAE buffer
2. Microwave 2 min at 800W (40-60 sec for low melt)
3. Cool to 55°C before adding nucleic acid stains
4. Pour into casting tray with 6-8mm comb
    

3.2 Voltage Optimization

Run at 5-8 V/cm for large fragments (>10kb) or 10-15 V/cm for small fragments (<1kb). Maintain buffer temperature below 35°C.

4. Industry Case Studies

4.1 ABC Biotech – CRISPR Validation

Challenge: Required high-resolution separation of 150-300bp CRISPR-edited fragments.
Solution: 2% UltraPure Agarose in TBE buffer with 7V/cm for 90min
Outcome: Achieved 5bp resolution between edited sequences.

4.2 PharmaCorp – Vaccine QC

Challenge: Detecting plasmid DNA impurities in mRNA vaccines.
Solution: 0.8% EEO-modified gel with alkaline running conditions
Outcome: Identified 0.01% contaminant DNA reliably.

4.3 University Core Lab – Metagenomics

Challenge: Simultaneous analysis of diverse microbial DNA sizes.
Solution: 1.2% agarose with pulsed-field gradient parameters
Outcome: Enabled single-gel analysis of 500bp-25kb fragments.

5. Technical Support & Inquiry

Our ISO 9001-certified production ensures batch-to-batch consistency with full COA documentation. Request custom certifications:

• DNase/RNase Test Reports
• Pyrogenicity Assays
• Sterility Validation

Contact our application scientists:
Email: info@vivalr.com
Tel: (86) 15866781826

Technical Documents Available Upon Request:

• Material Safety Data Sheet (MSDS)
• Gel Migration Rate Calculator
• Buffer Compatibility Chart