6-carboxyfluorescein cas 3301-79-9

Table of Contents

• Product Overview
• Specifications & Comparative Analysis
• Applications in Life Sciences
• Usage Guidelines
• Case Studies
• Request a Quote

6-Carboxyfluorescein (CAS 3301-79-9): Product Overview

6-Carboxyfluorescein (6-FAM) is a fluorescent dye widely used as a tracer in molecular biology applications. With CAS number 3301-79-9, this xanthene derivative exhibits strong green fluorescence (λ_ex 492 nm, λ_em 517 nm) and is particularly valued for its water solubility due to the carboxylic acid functional group.

Key Parameters

Comparative Analysis with Similar Fluorescent Dyes

Core Applications in Biotechnology

1. DNA Sequencing & Fragment Analysis

As a primer label in automated Sanger sequencing, 6-carboxyfluorescein enables multiplex detection with minimal spectral overlap.

2. Real-Time PCR (qPCR)

Used in TaqMan probes for 5′ nuclease assays due to its stable fluorescence across thermal cycling conditions.

3. Flow Cytometry

Conjugated to antibodies for cell surface marker detection with excitation compatibility with 488 nm lasers.

4. Immunoassays

ELISA and lateral flow test development using streptavidin-6-FAM conjugates for sensitive detection.

Optimal Usage Protocol

Step 1: Solubilization

Dissolve in 0.1M carbonate buffer (pH 8.5) at 10 mM concentration. Vortex for 5 minutes.

Step 2: Conjugation

Activate with EDC/NHS chemistry at 1:5 molar ratio (dye:protein) for 2 hours at 4°C.

Step 3: Purification

Use PD-10 desalting columns to remove unconjugated dye. Confirm labeling efficiency via UV-Vis (A₄₉₂/A₂₈₀ >0.8).

Documented Case Studies

Case Study 1: High-Throughput SNP Genotyping

Client: Genomics Core Facility, European Research Institute
Application: 6-FAM labeled probes in 384-well TaqMan assays for Alzheimer’s disease risk alleles
Result: Achieved 99.2% call rate with CV <2% across 10,000 samples

Case Study 2: Point-of-Care Diagnostic Development

Client: Asian IVD Manufacturer
Application: Lateral flow anti-dengue IgM detection using 6-FAM-streptavidin conjugates
Result: 95% sensitivity at 0.1 IU/mL cutoff, stable at 40°C/75% RH for 18 months

Technical & Commercial Inquiry

For batch-specific COA, bulk pricing (1g to 1kg scale), or custom conjugation services:

Email: info@vivalr.com
Phone: (86) 15866781826

Request quote code: FLUOR-6FAM-2025