HEPES Cas 7365-45-9

HEPES (Cas 7365-45-9) Product Guide

Table of Contents

• 1. Product Overview & Specifications
• 2. Key Applications & Usage
• 3. Comparative Analysis with Other Buffers
• 4. Usage Guidelines & Precautions
• 5. Case Studies
• 6. Contact for Inquiries

1. Product Overview & Specifications

HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), CAS 7365-45-9, is a zwitterionic buffer widely used in biological research.

2. Key Applications & Usage

2.1 Cell Culture Applications

Maintains pH stability in CO₂-free environments for mammalian cell cultures.

2.2 Molecular Biology

Used in PCR buffers, protein crystallization, and electrophoresis.

2.3 Biochemical Assays

Minimal metal ion chelation preserves enzyme activity in kinase studies.

3. Comparative Analysis with Other Buffers

4. Usage Guidelines & Precautions

4.1 Solution Preparation

Dissolve in distilled water at 0.5-1M concentration, adjust pH with NaOH/HCl.

4.2 Storage Conditions

Store powder at 2-8°C; solutions stable for 6 months at 4°C.

5. Case Studies

5.1 Biotech Company A: Mammalian Cell Line Optimization

Challenge: 40% cell viability drop in CO₂-independent media.
Solution: 25mM HEPES buffer implementation.
Result: Viability improved to 92% with stable pH maintenance.

5.2 University Research Team: Protein Purification

Challenge: Enzyme degradation during purification.
Solution: HEPES-based lysis buffer (50mM, pH 7.4).
Result: 80% activity retention vs 45% with phosphate buffer.

6. Contact for Inquiries

Request custom formulations or bulk pricing:
Email: info@vivalr.com
Phone: (86) 15866781826