1. Product Overview & Specifications

Coelenterazine (CAS 55779-48-1) is a luminogenic substrate widely used in bioluminescence imaging, reporter gene assays, and marine biology research. Below is a comparative analysis of common variants:

2. Key Applications & Use Cases

2.1 Bioluminescence Imaging

Used as a substrate for in vivo imaging of tumors, gene expression, and cellular pathways in organisms ranging from zebrafish to murine models.

2.2 Reporter Gene Assays

Enables high-throughput screening for drug discovery by quantifying transcriptional activity via Renilla or Gaussia luciferase systems.

2.3 ATP Detection

Integrated into kits for ultrasensitive ATP quantification in microbiological and environmental samples.

2.4 Molecular Interaction Studies

Supports BRET (Bioluminescence Resonance Energy Transfer) assays for protein-protein interaction analysis.

3. Detailed Usage Instructions

3.1 Preparation of Stock Solution

1. Dissolve 1 mg Coelenterazine in 1 mL anhydrous ethanol (for Native variant).
2. Vortex for 30 seconds and centrifuge at 10,000 rpm for 2 minutes.
3. Aliquot and store at -20°C protected from light.

3.2 In Vivo Imaging Protocol

1. Inject 150 μg/kg body weight into the subject intraperitoneally.
2. Wait 5 minutes for substrate distribution.
3. Acquire images using IVIS Spectrum system with 1-minute exposure.

4. Real-World Customer Case Studies

Case Study 1: Oncology Research Lab (University of Cambridge)

Challenge: Tracking metastatic breast cancer cells in real-time.
Solution: Used Coelenterazine h (5 μM final concentration) with Gaussia luciferase-tagged cells.
Result: Achieved 12-hour continuous monitoring with 3x higher signal-to-noise ratio than previous methods.

Case Study 2: Biotech Startup (NexGen BioSolutions)

Challenge: High-throughput screening of 10,000+ compounds for kinase inhibition.
Solution: Implemented Coelenterazine hcp in 384-well microplate assays.
Result: Reduced assay time by 40% while maintaining Z’ factor >0.7.

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