Alizarin Complexone CAS 3952-78-1

Chemical Name: Alizarin complexone
Synonyms: Alizarin Fluorine Blue
CAS No.: 3952-78-1
Molecular Fomula: C19H15NO8
Molecular weight: 385.32
Appearance: White to yellow to orange or brown powder
Assay: 98%

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Alizarin Complexone CAS 3952-78-1: Comprehensive Guide

Table of Contents

Product Overview & Specifications

Alizarin Complexone (CAS 3952-78-1) is a metallochromic indicator widely used for the detection of calcium, aluminum, and other metal ions. Below are its key parameters:

Parameter Alizarin Complexone Alizarin Red S
CAS Number 3952-78-1 130-22-3
Molecular Formula C₁₉H₁₅NO₈ C₁₄H₇NaO₇S
Sensitivity to Ca²⁺ High (0.1-10 ppm) Moderate (1-50 ppm)
Applications Fluorometric assays, EDTA titrations Histological staining

Key Applications

  • Water Quality Testing: Detects calcium hardness in drinking water and industrial wastewater.
  • Biomedical Research: Quantifies calcium flux in cellular studies via fluorescence.
  • Industrial Processes: Monitors metal ion concentrations in cooling systems and boiler feedwater.

Usage Scenarios

Scenario 1: Calcium Detection in Water Treatment

  1. Prepare 0.1% Alizarin Complexone solution in ammonium acetate buffer (pH 4.5).
  2. Add 2 mL reagent to 100 mL water sample.
  3. Measure fluorescence at 580 nm (excitation 470 nm).

Scenario 2: Cell Biology Imaging

  1. Incubate tissue samples with 5 μM Alizarin Complexone for 30 mins.
  2. Rinse with PBS to remove unbound reagent.
  3. Visualize calcium deposits under fluorescence microscopy.

Client Case Studies

Case 1: Environmental Tech Co., Germany

  • Challenge: Accurate detection of sub-ppm calcium in ultrapure water.
  • Solution: Implemented fluorometric analysis with Alizarin Complexone.
  • Outcome: Achieved 95% accuracy vs. 78% with traditional titration.

Case 2: BioHealth Labs, USA

  • Challenge: Tracking real-time calcium signaling in neurons.
  • Solution: Used Alizarin Complexone for live-cell imaging.
  • Outcome: Reduced photobleaching by 40% compared to Fura-2.

Contact for Quotation & Technical Support

Email: info@vivalr.com
Phone: (86) 15866781826

Request a quote today for bulk orders (>100g) or discuss custom formulations tailored to your metal ion detection needs.

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