Hematoxylin (CAS 1412-19-7): Comprehensive Product Guide
Table of Contents
1. Product Specifications & Comparative Analysis
| Parameter | Hematoxylin (CAS 1412-19-7) | Standard Histological Stains |
|---|---|---|
| Chemical Name | Hematoxylin Hydrate | Eosin Y |
| Molecular Formula | C16H20O9 | C20H8Br4O5 |
| Molecular Weight | 356.325 g/mol | 691.86 g/mol |
| Melting Point | 200°C (decomposition) | 296°C |
| Density | 1.03 g/mL | 1.27 g/cm³ |
2. Detailed Applications
Hematoxylin (CAS 1412-19-7) serves as a cornerstone reagent in:
- Histopathology: Nuclear staining in H&E (Hematoxylin and Eosin) protocols
- Cytogenetics: Chromosome banding visualization
- Neuroscience: Myelin sheath staining in neural tissue analysis
- Botanical Studies: Plant tissue structure delineation
Specialized formulations include Delafield’s and Weigert’s solutions for enhanced staining precision in specific microscopy applications.
3. Usage Scenarios
Protocol Implementation:
- Prepare Harris Hematoxylin solution (2% w/v in ethanol)
- Deparaffinize tissue sections using xylene gradient
- Stain nuclei for 5-8 minutes at 25°C
- Differentiate in 1% acid alcohol solution
- Blue in Scott’s tap water substitute
Optimized for use with automated staining systems and compatible with most mounting media.
4. Client Success Stories
National Pathology Institute
Challenge: Required consistent nuclear contrast for high-throughput cancer screening
Solution: Implemented standardized hematoxylin protocols with pH-controlled differentiation
Outcome: 23% improvement in diagnostic accuracy with 98% batch-to-batch consistency
University Research Laboratory
Challenge: Needed archival tissue compatibility for retrospective studies
Solution: Custom hematoxylin formulation with enhanced oxidative stability
Outcome: Enabled analysis of 15-year-old specimens with comparable results to fresh samples
5. Contact for Technical Consultation
Email: info@vivalr.com
Phone: (86) 15866781826
Request product specifications sheets or schedule a virtual demonstration with our histochemistry specialists.
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