BIS-Tris Propane Cas 64431-96-5

Chemical Name: BIS-Tris Propane
Synonyms: B-T-Propane; 1,3-Bis[tris(hydroxymethyl)methylamino]propane
CAS: 64431-96-5
Molecular Formula: C11H26N2O6
Molecular Weight: 282.33

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BIS-Tris Propane (Cas 64431-96-5): Comprehensive User Guide

1. Product Overview and Specifications

BIS-Tris Propane (CAS 64431-96-5) is a high-purity biochemical buffer widely used in molecular biology and pharmaceutical research. Below are its key parameters:

Parameter BIS-Tris Propane Tris-HCl HEPES
pH Range 6.3–9.5 7.0–9.0 6.8–8.2
Metal Chelation High Low Moderate
Solubility (25°C) >500 g/L ~350 g/L ~250 g/L

2. Key Applications of BIS-Tris Propane

BIS-Tris Propane is essential in:

  • Protein Crystallography: Stabilizes pH during crystal growth.
  • Electrophoresis: Maintains consistent buffer conditions in SDS-PAGE.
  • Enzyme Assays: Reduces metal ion interference in kinetic studies.

3. Usage Guidelines and Protocols

Typical Workflow for SDS-PAGE:

  1. Prepare 1M stock solution by dissolving 209.2 g in 1L deionized water.
  2. Adjust pH to 7.0 using HCl or NaOH.
  3. Dilute to 0.1M working concentration with ultrapure water.

4. Case Studies: Real-World Applications

Case 1: Enhancing Protein Stability in Drug Formulation

A biotech firm achieved 30% higher protein shelf-life by replacing phosphate buffer with 0.2M BIS-Tris Propane (pH 8.0).

5. Client Success Stories

Client A: PharmaTech Solutions

Used BIS-Tris Propane to optimize mRNA vaccine purification, reducing process time by 22%.

Client B: University Research Lab

Improved DNA ligation efficiency by 40% using BIS-Tris Propane in restriction enzyme buffers.

6. Contact Us for Queries

Request a quote or technical support:

Email: info@vivalr.com

Tel: (86) 15866781826

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