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Specialized inBIS-Tris Propane Cas 64431-96-5and related product development and production

Chemical Name: BIS-Tris Propane
Synonyms: B-T-Propane; 1,3-Bis[tris(hydroxymethyl)methylamino]propane
CAS: 64431-96-5
Molecular Formula: C11H26N2O6
Molecular Weight: 282.33

Samples: Available / Stock: Sufficient
Estimated delivery time: 3-5 days

BIS-Tris Propane (Cas 64431-96-5): Comprehensive User Guide

Table of Contents

1. Product Overview and Specifications

BIS-Tris Propane (CAS 64431-96-5) is a high-purity biochemical buffer widely used in molecular biology and pharmaceutical research. Below are its key parameters:

Parameter BIS-Tris Propane Tris-HCl HEPES
pH Range 6.3–9.5 7.0–9.0 6.8–8.2
Metal Chelation High Low Moderate
Solubility (25°C) >500 g/L ~350 g/L ~250 g/L

2. Key Applications of BIS-Tris Propane

BIS-Tris Propane is essential in:

  • Protein Crystallography: Stabilizes pH during crystal growth.
  • Electrophoresis: Maintains consistent buffer conditions in SDS-PAGE.
  • Enzyme Assays: Reduces metal ion interference in kinetic studies.

3. Usage Guidelines and Protocols

Typical Workflow for SDS-PAGE:

  1. Prepare 1M stock solution by dissolving 209.2 g in 1L deionized water.
  2. Adjust pH to 7.0 using HCl or NaOH.
  3. Dilute to 0.1M working concentration with ultrapure water.

4. Case Studies: Real-World Applications

Case 1: Enhancing Protein Stability in Drug Formulation

A biotech firm achieved 30% higher protein shelf-life by replacing phosphate buffer with 0.2M BIS-Tris Propane (pH 8.0).

5. Client Success Stories

Client A: PharmaTech Solutions

Used BIS-Tris Propane to optimize mRNA vaccine purification, reducing process time by 22%.

Client B: University Research Lab

Improved DNA ligation efficiency by 40% using BIS-Tris Propane in restriction enzyme buffers.

6. Contact Us for Queries

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Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
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A 70+ R&D team led by Chinese and foreign doctors
150 pieces (sets) of R&D equipment, 120+ fume hoods
18 patents

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Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
It has successfully passed on-site inspections from the United States, Australia, Poland, Japan and domestic pharmaceutical company customers and institutions many times, providing high-quality one-stop services for R&D and production of new drugs around the world.

More than 150 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

About the company

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FAQ

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Whether to provide samples?

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Delivery time

Under normal circumstances, all items are in stock and will be shipped before 4 o'clock on the same day. Some products are produced to order, depending on the workshop situation and production schedule.

What is the production capacity?

At present, the company's workshop production equipment is mainly based on laboratories and pilot lines, with a maximum daily production capacity of 1.5 tons.

Can additional test indicators be added?

We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Chem-iso’s delivery rate is guaranteed to be 99.99%

5000+

square meter R&D center

150+

China(set) of R&D equipment

150+

Fume hood

180+

R&D team

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