Tricine CAS 5704-04-1

Table of Contents

• 1. Product Overview & Specifications
• 2. Key Applications in Life Sciences
• 3. Operational Guidelines & Safety Protocols
• 4. Case Studies & Industry Implementations
• 5. Technical Support & Inquiry

1. Product Overview & Specifications

Key Advantages: Low UV absorbance, minimal metal ion interference, and superior resolution for low-molecular-weight peptides (＜10 kDa) in electrophoresis.

2. Key Applications in Life Sciences

Core Applications:

• Protein Electrophoresis: Preferred buffer for Tricine-SDS-PAGE systems, enabling precise separation of polypeptides between 1-100 kDa.
• Enzyme Assays: Maintains stable pH in oxidoreductase and hydrolase studies (7.6–8.5).
• Cell Culture Media: Buffering component for pH-sensitive mammalian cell lines.
• Chromatography: Mobile phase additive in HPLC separations of biomolecules.

Emerging Uses:

• Nanoparticle synthesis stabilization
• CRISPR-Cas9 reaction optimization

3. Operational Guidelines & Safety Protocols

Preparation of 1M Stock Solution:

1. Dissolve 179.17 g Tricine in 800 mL ultrapure H₂O
2. Adjust to pH 8.0 with NaOH/HCl
3. Bring final volume to 1 L

Safety Data:

4. Case Studies & Industry Implementations

Case Study 1: Vaccine Development (Shanghai Research Institute)

• Challenge: Stabilize pH in mRNA-LNP formulations
• Solution: 25 mM Tricine buffer (pH 8.0)
• Outcome: 98% encapsulation efficiency maintained at 4°C for 6 months

Case Study 2: Proteomics Core Facility (University of California)

• Challenge: Resolve 2-5 kDa antimicrobial peptides
• Solution: Tricine-SDS-PAGE (16.5% T, 6% C)
• Outcome: Achieved baseline separation of 1 kDa isoforms

Case Study 3: Biopharma Manufacturing (Hunan Hepure Bio)

• Challenge: Reduce buffer-induced aggregation in mAbs
• Solution: 50 mM Tricine in purification steps
• Outcome: Aggregate content reduced from 3.2% to 0.8%

5. Technical Support & Inquiry

For batch-specific COA, bulk pricing (＞10 kg), or formulation support:

Lead Time: 3-5 business days for standard orders (1 kg–100 kg)