1. Product Overview & Specifications

HEPPS (3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid), CAS 16052-06-5, is a zwitterionic biological buffer widely used in biochemical and molecular biology applications due to its stable pH range (7.8–8.8) and low metal ion binding.

Key Specifications

Comparative Analysis

2. Applications of HEPPS Buffer

Biochemistry & Molecular Biology

• Cell culture media for mammalian and insect cells
• Protein purification and crystallization
• PCR and electrophoresis

Pharmaceuticals

• Drug formulation stability testing
• Enzyme activity assays

Industrial Uses

• Diagnostic reagent preparation
• Cosmetic formulations

3. Usage Guidelines

Preparation Steps

1. Dissolve 23.8 g HEPPS in 800 mL deionized water.
2. Adjust pH to 8.0 with NaOH/HCl.
3. Bring volume to 1 L and filter-sterilize.

Best Practices

• Use fresh solutions for sensitive applications
• Avoid repeated freeze-thaw cycles

4. Case Studies

Lab Use: Mammalian Cell Culture

A research team achieved 95% cell viability using 25 mM HEPPS in DMEM media at pH 8.0 for hybridoma cell lines.

Industrial Use: Diagnostic Kits

HEPPS demonstrated superior stability in lateral flow assays compared to phosphate buffers during accelerated aging tests.

5. Customer Success Stories

BioGen Innovations (Shanghai)

Challenge: Protein aggregation during purification
Solution: 50 mM HEPPS buffer at pH 8.5
Result: 40% increase in soluble protein yield

PharmaSolutions Inc. (Boston)

Challenge: Drug formulation pH drift
Solution: HEPPS-based stabilization
Result: 12-month shelf-life stability achieved

6. Request a Quote

Contact our technical team for customized buffer solutions:

info@vivalr.com
(86) 15866781826