Bromocresol Purple (CAS 115-40-2): Comprehensive User Guide & Applications
Table of Contents
1. Product Overview & Parameter Comparison
Bromocresol Purple (CAS 115-40-2) is a triarylmethane dye widely used as a pH indicator. Key specifications include:
| Parameter | Bromocresol Purple | Bromothymol Blue | Phenol Red |
|---|---|---|---|
| CAS Number | 115-40-2 | 76-59-5 | 143-74-8 |
| pH Range | 5.2 (yellow) – 6.8 (purple) | 6.0-7.6 | 6.8-8.4 |
| Molecular Weight | 540.22 g/mol | 624.38 g/mol | 354.38 g/mol |
| Applications | Microbiology, Titration | Aquatic pH monitoring | Cell culture |
2. Detailed Applications
2.1 Microbiology
Acts as pH indicator in culture media for bacterial identification (e.g., MacConkey agar).
2.2 Analytical Chemistry
Used in acid-base titrations for precise endpoint detection between pH 5.2-6.8.
2.3 Medical Diagnostics
Component of urinalysis strips for detecting renal tubular acidosis.
3. Usage Scenarios & Best Practices
3.1 Preparation of 0.04% Solution
1. Dissolve 0.1g in 16.6ml 0.01M NaOH
2. Dilute to 250ml with distilled water
3. Filter through 0.22μm membrane
3.2 Quality Control
Verify color transition using pH 5.0 and 7.0 buffer solutions monthly.
4. Real-World Client Case Studies
Case 1: PharmaTech Solutions (Germany)
Challenge: Required stable pH indicator for antibiotic susceptibility testing.
Solution: Implemented 0.02% bromocresol purple in MH agar.
Result: 15% improvement in zone edge clarity.
Case 2: OceanBio Research Center (USA)
Challenge: Needed marine microbial culture indicator.
Solution: Custom 0.03% formulation with salinity tolerance.
Result: Stable performance at 3.5% NaCl concentration.
5. Contact for Technical Support
For formulation guidance or bulk pricing:
Email: info@vivalr.com
Tel: (86) 15866781826
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