4-NITROQUINOLINE N-OXIDE

Table of Contents

• Product Specifications & Comparative Analysis
• Applications in Biomedical Research
• Usage Scenarios & Experimental Protocols
• Client Case Studies
• Procurement Guidance

1. Technical Specifications & Comparative Analysis

Key differentiators: Superior mutagenic potency (EC50 0.2-0.5μM in cell models) with stable N-oxide structure enhancing DNA adduct formation efficiency.

2. Research Applications

Primary Use Cases:

• Oral squamous cell carcinoma induction in murine models
• UV-mimetic DNA damage studies
• Chemoprevention agent screening

Mechanistic Profile:

Acts through ROS-independent pathway, causing G-T transversion mutations. Compatible with:

• 3D organoid culture systems
• High-throughput genotoxicity assays
• Xenograft model pretreatment

3. Experimental Implementation

Standard Protocol (In Vitro):

1. Prepare stock solution in DMSO (10mM)
2. Treat cells at 0.1-5μM concentration
3. Exposure duration: 24-72 hours

Optimization Tips:

• Combine with hypoxia chamber (5% O2) for enhanced effect
• Use with NQO1 inhibitors for pathway-specific studies

4. Verified Client Implementations

Case 1: University Cancer Center

• Application: Head/neck cancer model development
• Results: Achieved 92% tumor incidence rate in 8 weeks
• Protocol: 50μg/mL in drinking water

Case 2: Pharma R&D Team

• Application: CAR-T therapy enhancement
• Outcome: 3.2x increase in neoantigen presentation
• Dosage: 2μM pretreatment for 48h

5. Procurement Advisory

Critical purchasing considerations:

• Batch-specific NMR validation reports
• -20℃ cold chain compliance
• Custom solubilization services

Request Quotation:

Contact our technical team for:

• MSDS documentation
• Bulk pricing (100g+ orders)
• Custom mutagenesis kits

Email: info@vivalr.com
Tel: (86) 15866781826