Cholesterol Esterase Cas 9026-00-0

Product name: Cholesterol Esterase
Synonyms: Biocatalytics E3; Bucelipase alfa; Sterol esterase
CAS No.: 9026-00-0

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Cholesterol Esterase (CAS 9026-00-0) Comprehensive User Guide

1. Product Overview & Specifications

Parameter Details
CAS Number 9026-00-0
Molecular Formula C47H53NO15
Molecular Weight 87,192 g/mol
Density 1.4 g/cm³
Boiling Point 982.8°C (at 760 mmHg)
Activity ≥300 units/mg (hydrolyzes 1 µmol cholesterol ester/min at 37°C, pH 7.0)
Purity ≥90% (chromatographically purified)

2. Key Applications

  • Clinical Diagnostics: Critical component in LDL/HDL cholesterol detection kits for accurate lipid profiling.
  • Biochemical Research: Hydrolyzes cholesterol esters into free cholesterol and fatty acids for metabolic studies.
  • Pharmaceutical Development: Used in drug formulations targeting cholesterol absorption disorders.
  • Food Industry: Enhances lipid digestion analysis in functional food studies.

3. Usage Guidelines

Storage Conditions

  • Store at -20°C in lyophilized form
  • Reconstitute with 0.1 M phosphate buffer (pH 6.5-7.0)

Optimal Working Conditions

  • Temperature: 37-50°C
  • pH Range: 6.5-7.5
  • Stabilizers: 0.1% BSA recommended

4. Industry Applications

Industry Application Benefit
Medical Diagnostics Lipoprotein cholesterol quantification 99% accuracy in clinical test results
Biotechnology Enzyme cascade reactions 30% faster reaction kinetics

5. Client Case Studies

Case Study 1: Diagnostic Laboratory Optimization

Client: Leading Shanghai-based diagnostics company
Challenge: Required high-purity enzyme for FDA-approved cholesterol test kits
Solution: Implemented chromatography-purified Cholesterol Esterase (300 U/mg)
Outcome: Achieved 15% improvement in test sensitivity

Case Study 2: Pharmaceutical Research

Client: Wuhan pharmaceutical developer
Challenge: Needed stable enzyme formulation for lipid metabolism studies
Solution: Customized lyophilized preparation with BSA stabilizers
Outcome: 40% reduction in batch variability

6. Contact for Technical Support

For product specifications, bulk orders, or technical consultation:
Email: info@vivalr.com
Phone: (86) 15866781826

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