Pyruvate Kinase (CAS 9001-59-6): Comprehensive User Guide
Table of Contents
1. Product Overview & Specifications
Pyruvate Kinase (CAS 9001-59-6) is a critical enzyme in glycolysis, catalyzing the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, generating ATP and pyruvate.
| Parameter | Specification | Competitor A | Competitor B |
|---|---|---|---|
| CAS Number | 9001-59-6 | 9001-59-6 | 9001-59-6 |
| Source | Rabbit Muscle | Yeast | Recombinant E. coli |
| Activity | ≥350 units/mg | ≥200 units/mg | ≥400 units/mg |
| Purity | ≥95% (SDS-PAGE) | ≥90% | ≥98% |
2. Key Applications & Uses
Pyruvate Kinase is widely used in:
- Biochemical research (glycolysis pathway studies)
- Diagnostic kits for blood glucose monitoring
- Enzyme-coupled assays (NADH/NADPH detection)
- Pharmaceutical production (ATP regeneration systems)
3. Usage Guidelines & Protocols
Typical Reaction Conditions:
| Component | Concentration |
|---|---|
| Pyruvate Kinase | 2-5 units/mL |
| ADP | 1.0 mM |
| PEP | 0.5 mM |
4. Case Studies
Case 1: ATP Generation Assay
Researchers achieved 95% ATP yield improvement using our pyruvate kinase in mitochondrial energy metabolism studies.
5. Customer Success Stories
ABC Diagnostics:
“Using this pyruvate kinase reduced our diagnostic kit production time by 40% while maintaining 99% assay accuracy.”
XYZ Biotech:
“Batch-to-batch consistency exceeded our expectations, enabling GMP-compliant pharmaceutical manufacturing.”
6. Contact for Quotations
Request pricing or technical support:
Email: info@vivalr.com
Phone: (86) 15866781826
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