Fluorescein Isothiocyanate Isomer I (CAS 3326-32-7): Comprehensive Guide for Researchers & Buyers
Table of Contents
1. Product Overview & Specifications
| Parameter | FITC Isomer I | Alternative Isomers |
|---|---|---|
| CAS Number | 3326-32-7 | 3326-32-7 (Isomer I) |
| Molecular Formula | C21H11NO5S | Same formula, different spatial configuration |
| Excitation/Emission | 494/521 nm | ±3 nm variation |
| Purity Grade | ≥95% (HPLC) | 85-90% typical |
| Solubility | DMSO, DMF (>10 mg/mL) | Limited aqueous solubility |
2. Key Applications & Industry Uses
Primary Application Areas:
- Flow Cytometry Biomarker Tagging
- Immunofluorescence Microscopy
- Protein Conjugation Studies
- Cell Tracing & Migration Analysis
- Biosensor Development
3. Usage Guidelines & Best Practices
Standard Protocol:
- Prepare 0.1M carbonate buffer (pH 9.0)
- Dissolve FITC in anhydrous DMSO (10 mg/mL)
- Maintain 4:1 molar ratio (protein:FITC)
- React at 4°C for 8 hours protected from light
- Purify via dialysis or size exclusion chromatography
4. Practical Application Scenarios
Case Example: Antibody Labeling
Researchers at Stanford University successfully conjugated FITC Isomer I to anti-CD4 antibodies for T-cell population analysis, achieving 92% labeling efficiency with minimal protein aggregation.
5. Verified Customer Case Studies
Case Study 1: Cancer Research Institute
| Parameter | Details |
|---|---|
| Application | In vivo tumor cell tracking |
| Result | 72-hour continuous monitoring capability |
| Technical Support | Customized conjugation protocol provided |
Case Study 2: Diagnostic Kit Manufacturer
| Parameter | Details |
|---|---|
| Challenge | Low fluorescence signal in lateral flow assays |
| Solution | Optimized FITC:antibody ratio (3:1) |
| Outcome | 150% signal intensity improvement |
6. Procurement & Technical Support
Why Choose Our FITC Isomer I:
- Batch-to-batch consistency guaranteed
- Technical documents include:
- MSDS
- HPLC Certificate
- Custom Conjugation Protocols
Contact Our Experts:
Email: info@vivalr.com
Phone: (86) 15866781826
Available: Mon-Fri 8:00-17:00 (GMT+8)
Request Custom Quote:
Specify required quantities (1mg-1kg available), packaging needs (amber vials with desiccant), and any special certification requirements (GMP-grade available).
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