5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE DISODIUM SALT CAS 102185-33-1

5-Bromo-4-chloro-3-indolyl Phosphate Disodium Salt (CAS 102185-33-1) User Guide

Table of Contents

• 1. Product Specifications & Comparative Analysis
• 2. Applications in Biochemical Research
• 3. Step-by-Step Usage Protocols
• 4. Case Studies & Industry Applications
• 5. Client Success Stories
• 6. Technical Consultation & Quotation

1. Product Specifications & Comparative Analysis

2. Applications in Biochemical Research

Primary uses in molecular diagnostics:

• Western Blot: Visualize alkaline phosphatase (ALP)-conjugated antibodies
• ELISA: Quantitative measurement of ALP activity
• Histochemistry: Tissue-specific enzyme localization

3. Step-by-Step Usage Protocols

Western Blot Procedure:

1. Prepare 1 mg/ml stock solution in DMSO
2. Mix with NBT (0.3 mg/ml final concentration)
3. Incubate membranes for 5-30 min at RT

4. Case Studies & Industry Applications

Pharmaceutical QC: Used in batch testing of recombinant protein ALP activity with 98% correlation to HPLC results.

5. Client Success Stories

Case 1: GenScript (Nanjing) reduced Western blot background noise by 40% through optimized BCIP/NBT formulation (2023).

Case 2: University of Cambridge Neuroscience Department achieved 3x faster staining vs. traditional methods.

6. Technical Consultation & Quotation

Contact our biochemistry specialists:

• Email: info@vivalr.com
• Tel: (86) 15866781826