5-Aminofluorescein CAS 3326-34-9: Comprehensive User Guide

1. Product Overview & Specification Comparison

Basic Parameters

Comparative Analysis

2. Key Applications of 5-Aminofluorescein

Core Applications

• Biomolecular Labeling: Covalent conjugation with antibodies/proteins via amine-reactive crosslinkers
• Fluorescent Probes: Heavy metal detection (Cu²⁺, Fe³⁺) with detection limits <1 ppm
• Diagnostic Reagents: Lateral flow assay development for rapid medical testing
• Environmental Monitoring: Real-time tracking of pollutant migration in groundwater
• Photodynamic Therapy: Photosensitizer in experimental cancer treatments

3. Usage Scenarios & Experimental Protocols

Example 1: Metal Ion Detection

1. Prepare 10 mM stock solution in 0.1M NaOH
2. Mix with sample solution (1:100 v/v ratio)
3. Incubate 15 min at 25°C protected from light
4. Measure fluorescence at 514nm (excitation 490nm)
5. Quantify using pre-established calibration curve

Example 2: Cellular Imaging

1. Conjugate with targeting antibodies via NHS chemistry
2. Apply 1μg/mL conjugate to fixed cells for 30min
3. Wash 3x with PBS buffer (pH 7.4)
4. Image using 488nm laser excitation

4. Real-World Customer Case Studies

Case 1: BioTech Solutions Ltd.

Challenge: Required stable fluorescent marker for COVID-19 antigen test strips
Solution: 5-Aminofluorescein-based conjugate showed 40% higher brightness than alternatives
Result: Achieved 99.2% clinical specificity in 10,000+ test samples

Case 2: University Research Team

Challenge: Tracking nanoplastic absorption in marine organisms
Solution: 5-Aminofluorescein-tagged nanoparticles with 72h stability
Result: Published in Environmental Science & Technology (Impact Factor: 9.028)

5. Contact for Technical Support & Quotations

For bulk orders, custom conjugations, or technical documentation:
Email: info@vivalr.com
Tel: (86) 15866781826

Our experts provide:
– Free sample testing for qualified applicants
– Regulatory support (REACH, FDA DMF)
– Custom synthesis (scale: 10mg to 10kg)