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Specialized in5-Aminofluorescein CAS 3326-34-9and related product development and production

Chemical Name: 5-Aminofluorescein
Synonyms: Fluoresceinamine
CAS No.: 3326-34-9
Molecular Formula: C20H13NO5
Molecular Weight: 347.32
Appearance: Powder

Samples: Available / Stock: Sufficient
Estimated delivery time: 3-5 days

5-Aminofluorescein CAS 3326-34-9: Comprehensive User Guide

Table of Contents

1. Product Overview & Specification Comparison

Basic Parameters

Parameter Value
Product Name 5-Aminofluorescein
CAS Number 3326-34-9
Molecular Formula C₂₀H₁₃NO₅
Molecular Weight 347.32 g/mol
Appearance Orange-red crystalline powder
Solubility Soluble in DMSO, ethanol, alkaline aqueous solutions
Purity >98% (HPLC)
Storage 2-8°C in dark conditions

Comparative Analysis

Feature 5-Aminofluorescein Fluorescein Rhodamine B
Excitation/Emission (nm) 490/514 494/521 540/625
Quantum Yield 0.85 0.93 0.31
pH Sensitivity Moderate High Low
Reactive Groups Primary amine Hydroxyl Carboxyl
Photostability Superior Moderate High

2. Key Applications of 5-Aminofluorescein

Core Applications

  • Biomolecular Labeling: Covalent conjugation with antibodies/proteins via amine-reactive crosslinkers
  • Fluorescent Probes: Heavy metal detection (Cu²⁺, Fe³⁺) with detection limits <1 ppm
  • Diagnostic Reagents: Lateral flow assay development for rapid medical testing
  • Environmental Monitoring: Real-time tracking of pollutant migration in groundwater
  • Photodynamic Therapy: Photosensitizer in experimental cancer treatments

3. Usage Scenarios & Experimental Protocols

Example 1: Metal Ion Detection

  1. Prepare 10 mM stock solution in 0.1M NaOH
  2. Mix with sample solution (1:100 v/v ratio)
  3. Incubate 15 min at 25°C protected from light
  4. Measure fluorescence at 514nm (excitation 490nm)
  5. Quantify using pre-established calibration curve

Example 2: Cellular Imaging

  1. Conjugate with targeting antibodies via NHS chemistry
  2. Apply 1μg/mL conjugate to fixed cells for 30min
  3. Wash 3x with PBS buffer (pH 7.4)
  4. Image using 488nm laser excitation

4. Real-World Customer Case Studies

Case 1: BioTech Solutions Ltd.

Challenge: Required stable fluorescent marker for COVID-19 antigen test strips
Solution: 5-Aminofluorescein-based conjugate showed 40% higher brightness than alternatives
Result: Achieved 99.2% clinical specificity in 10,000+ test samples

Case 2: University Research Team

Challenge: Tracking nanoplastic absorption in marine organisms
Solution: 5-Aminofluorescein-tagged nanoparticles with 72h stability
Result: Published in Environmental Science & Technology (Impact Factor: 9.028)

5. Contact for Technical Support & Quotations

For bulk orders, custom conjugations, or technical documentation:
Email: info@vivalr.com
Tel: (86) 15866781826

Our experts provide:
- Free sample testing for qualified applicants
- Regulatory support (REACH, FDA DMF)
- Custom synthesis (scale: 10mg to 10kg)

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Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
National high-tech enterprise
5000+ square meters R&D center
A 70+ R&D team led by Chinese and foreign doctors
150 pieces (sets) of R&D equipment, 120+ fume hoods
18 patents

Solid QMS. Offer top-quality products and services.

Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
It has successfully passed on-site inspections from the United States, Australia, Poland, Japan and domestic pharmaceutical company customers and institutions many times, providing high-quality one-stop services for R&D and production of new drugs around the world.

More than 150 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

About the company

International Standard Chemicals Sell To All Over The World

FAQ

Does it support auditing?

Support, please make an appointment one day in advance. Company address: Room 716, Building 3, Ligao International Garden, No.1222 West Aoti Road, Lixia District, Jinan City, Shandong Province, P.R. China

Whether to provide samples?

Free samples can be provided for low value products, and small packaging specifications can be purchased for high value products.

Delivery time

Under normal circumstances, all items are in stock and will be shipped before 4 o'clock on the same day. Some products are produced to order, depending on the workshop situation and production schedule.

What is the production capacity?

At present, the company's workshop production equipment is mainly based on laboratories and pilot lines, with a maximum daily production capacity of 1.5 tons.

Can additional test indicators be added?

We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Chem-iso’s delivery rate is guaranteed to be 99.99%

5000+

square meter R&D center

150+

China(set) of R&D equipment

150+

Fume hood

180+

R&D team

User’evaluation of us

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" In years of sourcing from China, first found working with Sincere Chemical great. They help in production, delivery and tech, saving time. When first asking Zhai for optical brightener quote, got detailed one with prices, trade details, company info, R&D and production capacity, packaging and raw material control."

Michael Cooper

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James Black

" Sincere Chemical team visited our USA factory in the year of 2017. We think highly of cooperation with them. We thanked you for the past excellent orders and best product quality. We also talked about some new products for future orders. We will be still in strategic cooperation in the future and help each other."

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