Geneticin CAS 108321-42-2

Table of Contents

• Product Overview
• Key Specifications & Comparative Analysis
• Applications in Life Science Research
• Usage Guidelines & Best Practices
• Case Studies: Geneticin in Action
• Request Product Specifications & Pricing

Geneticin (G418) CAS 108321-42-2: Essential Antibiotic for Cell Selection

Geneticin (CAS 108321-42-2), commercially known as G418 Sulfate, is an aminoglycoside antibiotic widely used for selecting eukaryotic cells transfected with neomycin resistance genes. With molecular formula C20H40N4O10·2H2SO4 and molecular weight 692.71 g/mol, it inhibits protein synthesis by binding to 80S ribosomes.

Key Specifications & Comparative Analysis

Core Research Applications

• Stable cell line development (e.g., CHO, HEK293)
• Knockout/Knockin model validation
• Antibiotic resistance gene selection
• Viral vector production optimization

Optimized Usage Protocol

1. Prepare stock solution (50 mg/mL in sterile H2O)
2. Filter-sterilize using 0.22 μm membrane
3. Determine optimal concentration (100-1000 μg/mL)
4. Apply selective pressure for 10-14 days

Documented Success Cases

Case 1: Stable CHO Cell Line Development (Thermo Fisher Scientific)

Challenge: Achieve 95% transfection efficiency in Chinese Hamster Ovary cells
Solution: 14-day selection with 800 μg/mL Geneticin
Outcome: Stable monoclonal colonies obtained in 18 days

Case 2: CRISPR-Edited HEK293 Validation (Sigma-Aldrich)

Challenge: Eliminate non-edited cells in gene knockout model
Solution: Dual selection with 500 μg/mL Geneticin + 1 μg/mL Puromycin
Outcome: 99.7% pure edited cell population achieved

Case 3: Lentiviral Packaging Optimization (ATCC)

Challenge: Improve viral titer consistency in packaging cells
Solution: Continuous Geneticin selection at 400 μg/mL
Outcome: 3.5x increase in functional viral particles/mL

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