5-Bromo-4-Chloro-3-Indolyl β-D (CAS 114162-64-0) Ultimate User Guide
Table of Contents
1. Product Specifications & Comparative Analysis
2. Detailed Applications in Life Science Research
3. Step-by-Step Usage Protocols
4. Industry Application Scenarios
1. Product Specifications & Comparative Analysis
| Parameter | 5-Bromo-4-Chloro-3-Indolyl β-D | X-Gal | Blue-Gal |
|---|---|---|---|
| CAS Number | 114162-64-0 | 7240-90-6 | 157115-85-2 |
| Molecular Formula | C14H10BrClN2O5 | C14H15Br3N2O2 | C14H15BrCl2N2O2 |
| Detection Sensitivity | 0.1-1 ng β-gal | 1-10 ng β-gal | 5-50 ng β-gal |
| Chromogenic Speed | 30-60 min | 2-4 hr | 4-8 hr |
2. Detailed Applications in Life Science Research
Essential for:
- β-galactosidase reporter gene detection in eukaryotic/prokaryotic systems
- Blue-white screening in plasmid vector construction
- Mammalian cell transfection efficiency analysis
- Microbial β-gal activity quantification (OD420 measurement)
- High-throughput drug screening assays
3. Step-by-Step Usage Protocols
Standard Protocol:
- Prepare 20 mg/ml stock solution in DMF
- Add 40 μl per 100 mm culture dish
- Incubate at 37°C protected from light
- Monitor color development every 15 min
4. Industry Application Scenarios
Case A: Yeast two-hybrid system optimization
Case B: CRISPR-edited cell line validation
Case C: Industrial enzyme production monitoring
5. Verified Customer Success Stories
Client 1: BioTech Solutions Co., Ltd.
- Application: High-throughput antibody screening
- Result: Reduced false positives by 68% vs traditional markers
Client 2: PharmaCore Research Institute
- Application: Gene therapy vector QC
- Result: Achieved 99.7% transfection efficiency confirmation
6. Technical Consultation & Ordering Guidance
Contact our biochemistry specialists:
Email: info@vivalr.com
Tel: (86) 15866781826
Request:
- Custom formulation options
- Bulk order pricing (1kg+ quantities)
- MSDS & COA documentation
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