Table of Contents
- Product Overview
- Technical Specifications & Comparative Analysis
- Applications in Biochemical Research
- Usage Guidelines
- Industry Case Studies (2020-2024)
- Contact for Technical Consultation
2-Nitrophenyl-β-D-glucopyranoside (CAS 2816-24-2) Overview
Key Characteristics
| Parameter | Specification |
|---|---|
| Molecular Formula | C₁₂H₁₅NO₈ |
| Molecular Weight | 301.25 g/mol |
| Purity | ≥98% (HPLC) |
| Appearance | White to off-white crystalline powder |
| Solubility | Water: 50 mg/mL, DMSO: 100 mg/mL |
| Storage | 2-8°C in dark, anhydrous conditions |
Technical Comparison with Similar Substrates
| Substrate | Sensitivity | Reaction Rate | Price per gram |
|---|---|---|---|
| 2-Nitrophenyl-β-D-glucopyranoside | 0.2 μM detection | 1.8 U/mg/min | $220-280 |
| 4-Nitrophenyl-β-D-glucopyranoside | 0.5 μM detection | 1.2 U/mg/min | $180-240 |
| PNP-β-D-glucopyranoside | 1.0 μM detection | 0.8 U/mg/min | $150-200 |
Primary Research Applications
Enzyme Activity Assays
Used as chromogenic substrate for β-glucosidase detection with linear range 0.05-5.0 U/mL.
Inhibitor Screening
IC₅₀ determination for diabetes drug candidates targeting carbohydrate-active enzymes.
Microbial Identification
Differentiation of β-glucoside utilizing bacteria in clinical microbiology (90% specificity).
Optimized Protocol (2024 Revision)
Recommended Workflow
| Step | Condition | Duration |
|---|---|---|
| Substrate Prep | 50 mM phosphate buffer, pH 6.8 | 30 min |
| Enzyme Reaction | 37°C with 2 mM substrate | 15-60 min |
| Detection | 405 nm absorbance | N/A |
Documented Success Cases
Case 1: University of Cambridge (2022)
Application: Screening novel β-glucosidase mutants
Result: Identified 3 variants with 4x enhanced activity
Feedback: “Superior stability in continuous assays vs. alternatives”
Case 2: Roche Diagnostics (2023)
Application: Urinary tract infection pathogen detection
Result: 92% clinical sensitivity achieved
Feedback: “Critical for our rapid diagnostic platform”
Technical & Commercial Inquiry
For batch-specific COA, bulk pricing (10g+ orders), or custom conjugate services:
Email: info@vivalr.com
Phone: (86) 15866781826
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