Chemical Name: Thymol Blue
CAS No.: 76-61-9
Molecular Fomula: C27H30O5S
Molecular weight: 466.59
Appearance: Dark green to dark brown solid
Assay: ≥99%
Thymol Blue (CAS 76-61-9) is a pH-sensitive triphenylmethane dye widely used as a biochemical indicator. Below is a technical comparison with similar indicators:
Parameter | Thymol Blue | Phenolphthalein | Bromocresol Green |
---|---|---|---|
pH Range | 1.2–2.8 (red-yellow) 8.0–9.6 (yellow-blue) |
8.2–10.0 | 3.8–5.4 |
Molecular Weight | 466.59 g/mol | 318.32 g/mol | 698.02 g/mol |
Thymol Blue serves as a dual-range indicator for strong acid-strong base (1st transition) and weak acid-strong base (2nd transition) titrations.
Used in cell culture media preparation and protein electrophoresis due to its non-toxic nature at low concentrations.
Dissolve 0.1g Thymol Blue in 21.5ml 0.01M NaOH + 250ml ethanol. Filter before use for optimal clarity.
A chemical plant achieved 0.05pH unit accuracy using Thymol Blue in pretreatment processes, reducing sensor calibration costs by 40%.
Implemented Thymol Blue in QC labs for injectable medications, reducing false-positive results by 22% through improved endpoint detection.
Chemistry Department reported 98% student success rate in titration experiments after switching to Thymol Blue-based protocols.
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