Collagenase CAS 9001-12-1

Chemical name: Collagenase
CAS.NO: 9001-12-1
Molecular weight: 849.4494
Appearance: light brown to brown powder

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Collagenase CAS 9001-12-1: Comprehensive User Guide (2025 Edition)

Table of Contents

1. Product Specifications & Comparative Analysis

Parameter Collagenase Type I Collagenase Type II Collagenase Type IV
CAS Number 9001-12-1 9001-12-1 9001-12-1
Activity Range 125-250 CDU/mg 300-500 CDU/mg 400-600 CDU/mg
Optimal pH 6.0-7.5 6.5-7.8 7.0-8.0
Thermal Stability 25-37°C 30-42°C 35-45°C

2. Key Applications in Biomedical Research

2.1 Tissue Dissociation Applications

  • Primary cell isolation (hepatocytes, cardiomyocytes)
  • Adipose-derived stem cell separation
  • Tumor tissue processing for cancer research

2.2 Wound Care Innovations

  • Debridement of burn wounds
  • Chronic ulcer treatment formulations
  • Bioactive wound dressing production

3. Operational Protocols & Best Practices

3.1 Standardized Digestion Protocol

  1. Prepare 0.1-0.3% w/v solution in HBSS
  2. Maintain temperature at 37±1°C
  3. Monitor digestion every 15 minutes

4. Industry Case Studies

Case Study 1: Biopharmaceutical Production

Client: Shanghai Biologics Co., Ltd. (2024)
Application: Large-scale vaccine production using collagenase-digested Vero cells
Result: 38% increase in cell yield with 99.2% viability

Case Study 2: Regenerative Medicine

Client: Boston Tissue Engineering Institute (2023)
Application: Decellularization of porcine heart valves
Result: Complete ECM preservation with 0% residual cellular material

5. Technical Support & Purchasing Guidance

Quality Assurance Parameters

  • Sterility: ≤1 CFU/10ml
  • Endotoxin: <0.1 EU/mg
  • Protease Activity: 150-450 PZ U/mg

Contact Our Specialists

For bulk orders or technical queries:
Email: info@vivalr.com
Tel: (86) 15866781826

Request Custom Formulation

  • Available in lyophilized powder or liquid formats
  • Customized activity levels (100-800 CDU/mg)
  • GMP-grade options available
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