Chemical Name: 2,3,5-Triphenyltetrazolium chloride
Synonyms: 2,3,5-Triphenyl-2H-tetrazolium chloride; Tetrazolium Red
CAS No.: 298-96-4
Molecular Fomula: C19H15ClN4
Molecular weight: 449.62
Appearance: White powder
Assay: ≥98.0%
CAS No.: 298-96-4
Molecular Formula: C19H15ClN4
Molecular Weight: 334.8 g/mol
Appearance: White to pale yellow crystalline powder
Melting Point: 243–250°C
Solubility: Soluble in water, ethanol, and acetone; insoluble in ether
Storage: Store in airtight containers at 2–8°C, protected from light and moisture.
Parameter | TTC (≥98%) | Standard Grade |
---|---|---|
Purity | ≥98% | ≥95% |
Water Content | ≤0.5% | ≤1.0% |
Residue on Ignition | ≤0.1% | ≤0.5% |
Solution Preparation:
Dissolve 0.1g TTC in 10mL ethanol (1% w/v). Filter through 0.22µm membrane for sterility.
Safety Protocols:
• Use PPE (gloves, goggles)
• Avoid contact with acids/reducing agents
• Dispose per local hazardous waste regulations
Case 1: Microbiology Labs
Added 0.5% TTC to agar media to differentiate viable bacterial colonies (red coloration) from inert particles.
Case 2: Pharma QC
Applied in USP/EP-compliant steroid assays with ≤2% RSD accuracy.
Shanghai HuaLan Chemical Co., Ltd.
- Integrated TTC in antibiotic potency testing, achieving 99.2% interlab consistency.
- Reduced false-positive rates by 40% in microbial limit tests.
Eurofins Central Laboratory
- Implemented TTC-based pesticide screening, detecting 0.01ppm residues in grains.
- Shortened analytical workflow by 35%.
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