2,3,5-Triphenyltetrazolium Chloride CAS 298-96-4

1. Product Overview & Specifications

CAS No.: 298-96-4
Molecular Formula: C₁₉H₁₅ClN₄
Molecular Weight: 334.8 g/mol
Appearance: White to pale yellow crystalline powder
Melting Point: 243–250°C
Solubility: Soluble in water, ethanol, and acetone; insoluble in ether
Storage: Store in airtight containers at 2–8°C, protected from light and moisture.

Comparative Specifications

2. Key Applications

• Biochemical Analysis: Quantify dehydrogenase activity via colorimetric reduction (colorless→red).
• Pharmaceutical Testing: Detect corticosteroids in drug formulations using tetrazolium blue method.
• Food Safety: Identify microbial contamination in food samples through selective staining.
• Agrochemical Research: Analyze pesticide residues with high sensitivity.
• Material Science: Titrate boranes (diborane, pentaborane) in synthetic processes.

3. Operational Guidelines

Solution Preparation:
Dissolve 0.1g TTC in 10mL ethanol (1% w/v). Filter through 0.22µm membrane for sterility.

Safety Protocols:
• Use PPE (gloves, goggles)
• Avoid contact with acids/reducing agents
• Dispose per local hazardous waste regulations

4. Industry Use Cases

Case 1: Microbiology Labs
Added 0.5% TTC to agar media to differentiate viable bacterial colonies (red coloration) from inert particles.

Case 2: Pharma QC
Applied in USP/EP-compliant steroid assays with ≤2% RSD accuracy.

5. Client Success Stories

Shanghai HuaLan Chemical Co., Ltd.
– Integrated TTC in antibiotic potency testing, achieving 99.2% interlab consistency.
– Reduced false-positive rates by 40% in microbial limit tests.

Eurofins Central Laboratory
– Implemented TTC-based pesticide screening, detecting 0.01ppm residues in grains.
– Shortened analytical workflow by 35%.

6. Contact for Technical Support

Request quotes or protocol optimization guidance:
info@vivalr.com
(86) 15866781826