Disodium 4-Nitrophenylphosphate CAS 4264-83-9
Chemical Name: Disodium 4-Nitrophenylphosphate
Synonyms: 4-Nitrophenyl phosphate disodium salt; 4-Nitrophenyl disodium orthophosphate; PNPP
CAS No.: 4264-83-9
Molecular Formula: C6H7NNaO6P
Molecular Weight: 243.09
Appearance: Flaky
Disodium 4-Nitrophenylphosphate (CAS 4264-83-9) User Guide
Table of Contents
1. Product Specifications & Comparative Analysis
| Parameter | Specification |
|---|---|
| CAS No. | 4264-83-9 |
| Molecular Formula | C6H4NNa2O6P |
| Molecular Weight | 263.05 (Anhydrous) 371.14 (Hexahydrate) |
| Appearance | White to pale yellow crystalline powder |
| Melting Point | 300°C |
| Solubility | Water-miscible (1 tablet dissolves in H2O) |
| Stability | Light/moisture-sensitive; decomposes above 25°C |
| Absorption Peak | 310 nm (native), 405 nm (reaction product) |
Comparative Advantages vs. Similar Substrates
| Feature | PNPP | OPD | TMB |
|---|---|---|---|
| Sensitivity | High (405 nm detection) | Moderate | High |
| Reaction Termination | 3N NaOH | H2SO4 | Acid |
| Storage | -20°C, desiccated | 4°C | RT |
2. Applications & Functional Mechanisms
Primary Use: Chromogenic substrate for alkaline/acid phosphatase assays in:
- ELISA-based diagnostic kits
- Pharmaceutical QC for enzyme activity validation
- Biochemical research (enzyme kinetics, inhibitor screening)
Reaction Workflow
- Substrate hydrolysis by phosphatases releases p-nitrophenol
- Yellow coloration intensity correlates with enzyme activity
- Quantification at 405 nm with linear range 0.1-10 mM
3. Operational Guidelines & Best Practices
Protocol Summary
| Step | Condition | Note |
|---|---|---|
| Reconstitution | 1 mg/mL in assay buffer | Prepare fresh |
| Incubation | 37°C, 5-30 min | Monitor color development |
| Termination | 3N NaOH (1:1 v/v) | Stabilizes signal for 2h |
Critical Handling Notes
- Avoid repeated freeze-thaw cycles
- Use amber vials for light-sensitive storage
- Discard precipitated solutions
4. Industry Use Cases
Diagnostic Manufacturing
Integrated into automated immunoassay platforms for:
- Hepatobiliary disease panels
- Bone metabolism marker tests
Biopharma R&D
High-throughput screening of phosphatase-targeted drug candidates with:
- Z´ factor >0.7 in 384-well formats
- CV <8% across replicates
5. Client Success Stories
Case 1: European Pharma Leader
- Challenge: Unstable phosphatase signals in lead optimization
- Solution: PNPP-based kinetic assays with real-time monitoring
- Outcome: 40% reduction in false positives
Case 2: Asian CRO
- Challenge: Batch variability in clinical trial kits
- Solution: Standardized PNPP lots with COA tracking
- Outcome: 99.2% inter-assay consistency achieved
6. Technical Support & Inquiry
Request bulk pricing, COA, or formulation guidance:
Email: info@vivalr.com
Tel: (86) 15866781826
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