Table of Contents
- Product Overview & Specifications
- Key Applications of Giemsa Stain
- Step-by-Step Usage Protocol
- Industry Case Studies (2022-2024)
- Technical Consultation & Ordering
Giemsa Stain (CAS 51811-82-6) Product Specifications
Technical Parameters Comparison
| Parameter | Giemsa Stain | Wright Stain | Leishman Stain |
|---|---|---|---|
| CAS Number | 51811-82-6 | 68988-92-1 | 12627-53-1 |
| Optimal pH Range | 6.4-6.8 | 6.5-7.0 | 6.2-6.6 |
| Staining Time | 15-30 mins | 5-10 mins | 10-15 mins |
| Nuclear Detail | Superior | Moderate | Good |
Chemical Composition
- Azure B Eosinate: 45-55%
- Methylene Blue: 30-40%
- Eosin Y: 10-15%
- Stabilizers: <3%
Diagnostic & Research Applications
Medical Diagnostics
- Malaria parasite detection in blood smears
- Bone marrow differential counts
- Helicobacter pylori identification
Life Science Research
- Chromosome banding analysis
- Apoptosis detection in cell cultures
- Pathogen identification in tissue sections
Standard Operating Procedure
Blood Smear Protocol
- Prepare 10% Giemsa working solution in pH 6.8 buffer
- Fix slides in absolute methanol (2 mins)
- Stain at room temperature (20 mins)
- Differentiate in pH 6.8 buffer (1 min)
- Air dry vertically
Troubleshooting Guide
| Issue | Solution |
|---|---|
| Over-stained nuclei | Reduce staining time by 5 mins |
| Precipitate formation | Filter through Whatman #1 paper |
Client Success Stories
Case Study 1: XYZ Diagnostics Lab (2023)
- Challenge: 15% false negatives in malaria screening
- Solution: Optimized Giemsa staining protocol
- Result: 99.2% detection accuracy achieved
Case Study 2: ABC Research Institute (2024)
- Application: Chromosome analysis in leukemia
- Outcome: Clear G-banding patterns achieved
- Throughput: 200+ slides/day processed
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