Nitrotetrazolium blue chloride cas 298-83-9

Nitrotetrazolium Blue Chloride (CAS 298-83-9): Comprehensive Guide & Applications

Table of Contents

• 1. Product Overview & Specifications
• 2. Key Applications & Use Cases
• 3. Technical Parameters & Comparisons
• 4. Safety & Handling Guidelines
• 5. Customer Case Studies
• 6. Request Custom Quotes

1. Product Overview & Specifications

Nitrotetrazolium Blue Chloride (CAS 298-83-9) is a water-soluble tetrazolium salt widely used as a redox indicator in biochemical assays. Key specifications include:

2. Key Applications & Use Cases

Primary industrial and research applications:

• Cell Viability Assays: Detects mitochondrial activity via NADPH-dependent reduction to formazan.
• Microbial Identification: Differentiates bacterial species in diagnostic kits (e.g., API strips).
• Superoxide Detection: Quantifies O₂^– in phagocytosis studies.
• Enzyme Activity Tests: Used in dehydrogenase and reductase assays.

3. Technical Parameters & Comparisons

4. Safety & Handling Guidelines

• Storage: 2-8°C in airtight amber vials
• Hazard Codes: H315-H319-H335 (Skin/Eye irritation, respiratory sensitization)
• PPE Requirements: Lab coat, nitrile gloves, safety goggles
• Disposal: Follow local regulations for hazardous organic salts

5. Customer Case Studies

Case 1: University Medical Research Lab

Challenge: Required high-throughput screening of antifungal agents.
Solution: Implemented NBT-based superoxide detection in 96-well plates.
Result: 40% reduction in assay time vs. MTT; 92% correlation with flow cytometry data.

Case 2: Pharmaceutical QC Department

Challenge: Validate microbial limits testing per USP <61>.
Solution: Used NBT chloride in rapid species differentiation protocol.
Result: Achieved 99.3% accuracy in 8hr identification vs. traditional 24hr methods.

6. Request Custom Quotes

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